Annurca apple is a southern Italian variety. The fruit is picked unripe, "green" and placed for 20–40 days in special boxes called “melai”; then, the apples are sun-exposed and when the exposed side turns red, the fruit is rotated in order to give its opposite side the chance to turn red. We have explored the effects of Annurca polyphenol extract (APE) on human breast cancer cells (MCF-7). Material & Methods: Annurca apple flesh was homogenized and the total polyphenolic content of APE was assessed. Cells were treated with different concentration of APE for 24 and 48 hours and we have studied the APE effects on proliferation in MCF-7 cells. We have observed changes in cell morphology. High concentrations APE have acted as prooxidant, increasing thiobarbituric acid-reactive species. We have found that APE inhibits MCF-7 cells proliferation by inducing G2/M cell cycle arrest and apoptosis. Our data indicate that APE acts as a potent prooxidant and antiproliferative agent, that Annurca is an excellent source of bioactive phytochemicals that may provide special nutraceutical benefits and provides a rationale for APE potential use in the development of novel therapeutics towards breast cancer.
|Titolo:||INHIBITION OF BREAST CANCER CELL PROLIFERATION BY ANNURCA APPLE POLYPHENOLS|
|Autori interni:||D'ANGELO, Stefania|
|Data di pubblicazione:||2017|
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