Polyphenols have been demonstrated to have clear antioxidant activities in vitro. However, in complex biological systems, they exhibit additional properties which are yet poorly understood. Apple is among the most consumed fruits worldwide, and several studies suggest that apple polyphenols could play a role in the prevention of degenerative diseases. The present study aimed at evaluating the “Annurca” apple polyphenol extract (APE) effects both proliferation and apoptosis on HaCaT cells. The data indicate that apple polyphenolic compounds had significant antiproliferative action on HaCaT cells. FACS analysis showed that APE induced cell apoptosis in a dose-dependent manner. Moreover, apple polyphenols induced apoptosis in epithelial cells by triggering death receptor-associated extrinsic pathway p53-independent. Apple polyphenol extract was also capable of inducing morphological changes as evidenced by nuclear condensation. The cellular, morphological and molecular data unequivocally demonstrated that induction of cellular apoptosis was mainly responsible for the previously observed anti-proliferation induced APE on HaCaT keratinocytes. Our experimental results suggest that apple polyphenols are a promising source from which a natural-based topical agent could be developed for skin diseases treatment.

Effect of apple polyphenols on human HaCaT keratinocytes proliferation

D'ANGELO, Stefania;
2012

Abstract

Polyphenols have been demonstrated to have clear antioxidant activities in vitro. However, in complex biological systems, they exhibit additional properties which are yet poorly understood. Apple is among the most consumed fruits worldwide, and several studies suggest that apple polyphenols could play a role in the prevention of degenerative diseases. The present study aimed at evaluating the “Annurca” apple polyphenol extract (APE) effects both proliferation and apoptosis on HaCaT cells. The data indicate that apple polyphenolic compounds had significant antiproliferative action on HaCaT cells. FACS analysis showed that APE induced cell apoptosis in a dose-dependent manner. Moreover, apple polyphenols induced apoptosis in epithelial cells by triggering death receptor-associated extrinsic pathway p53-independent. Apple polyphenol extract was also capable of inducing morphological changes as evidenced by nuclear condensation. The cellular, morphological and molecular data unequivocally demonstrated that induction of cellular apoptosis was mainly responsible for the previously observed anti-proliferation induced APE on HaCaT keratinocytes. Our experimental results suggest that apple polyphenols are a promising source from which a natural-based topical agent could be developed for skin diseases treatment.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11367/27847
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