Introduction: lemon peel polyphenols extract (LPE) from lemon (Citrus limon) agroindustry’s wastes, shows antioxidant and anti-cancer properties in various cancer cells (1). Our aim was to explore the LPE protective effect using an in vitro inflammatory model consisting of Interleukin-6 (IL-6) treated human gastric adenocarcinoma (MKN-28 and AGS) and primary colon cancer (T88 and T93) cells. We evaluated the LPE effect on IL-6-dependent cell invasiveness and matrix metalloproteinases (MMP)-9/2 expression. Materials and Methods: cells pretreated with LPE (1) at non-cytotoxic concentrations (0.5 or 1 µg/ml gallic acid equivalent, 6 h), and then exposed to IL-6 (50 ng/ml, 24 h) were analyzed for: cell migration (wound assay) and invasiveness (Boyden chamber assay); MMP-9/2 mRNA expression level (q-PCR); gelatinolytic activity (gelatin zymography) and protein expression level (SDS-PAGE and Western blotting). Results: LPE pretreatment reduces the invasiveness of these cancer cells through the inhibition of IL-6-induced MMP-9/2 up-regulation (2,3). Conclusions: LPE represents a cancer protective agent and a green strategy for residual biomass recovery that refocus economic and environmental aspects of agro-food industry. References: 1. Di Donato et al. 2018, Waste Biomass Valorization, 9, 1567. 2. Pagliara et al. 2019, Biomolecules, 9, 833. 3. Pagliara et al. 2021, Molecules, 26, 7076.

Lemon Peel Polyphenol Extract reduces Interleukin-6 dependent Invasiveness and Matrix Metalloproteinase-9/2 Expression in human gastric and primary colon carcinoma cells.

ROSARIA ARCONE
;
VALENTINA PAGLIARA;ROSARITA NASSO;PAOLA DI DONATO;ANTONIO D’ERRICO;MARIOROSARIO MASULLO.
2022

Abstract

Introduction: lemon peel polyphenols extract (LPE) from lemon (Citrus limon) agroindustry’s wastes, shows antioxidant and anti-cancer properties in various cancer cells (1). Our aim was to explore the LPE protective effect using an in vitro inflammatory model consisting of Interleukin-6 (IL-6) treated human gastric adenocarcinoma (MKN-28 and AGS) and primary colon cancer (T88 and T93) cells. We evaluated the LPE effect on IL-6-dependent cell invasiveness and matrix metalloproteinases (MMP)-9/2 expression. Materials and Methods: cells pretreated with LPE (1) at non-cytotoxic concentrations (0.5 or 1 µg/ml gallic acid equivalent, 6 h), and then exposed to IL-6 (50 ng/ml, 24 h) were analyzed for: cell migration (wound assay) and invasiveness (Boyden chamber assay); MMP-9/2 mRNA expression level (q-PCR); gelatinolytic activity (gelatin zymography) and protein expression level (SDS-PAGE and Western blotting). Results: LPE pretreatment reduces the invasiveness of these cancer cells through the inhibition of IL-6-induced MMP-9/2 up-regulation (2,3). Conclusions: LPE represents a cancer protective agent and a green strategy for residual biomass recovery that refocus economic and environmental aspects of agro-food industry. References: 1. Di Donato et al. 2018, Waste Biomass Valorization, 9, 1567. 2. Pagliara et al. 2019, Biomolecules, 9, 833. 3. Pagliara et al. 2021, Molecules, 26, 7076.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11367/110739
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