Aspartate aminotranferase from the thermophilic bacterium Thermus aquaticus has been purified to homogeneity. The enzyme is pyridoxal-5-phosphate dependent and is composed of two subunits having an Mr 44000 each. T. aquaticus AspAT shows a pI of 4.5 and is a thermophilic enzyme exhibiting its maximum activity at 80 degrees C in the pH range 7-8. It is also resistant against denaturation to heat and several chemical agents.

Aspartate aminotranferase from the thermophilic bacterium Thermus aquaticus YT-1. Functional characterisation and stability

MASULLO, Mariorosario;
1996

Abstract

Aspartate aminotranferase from the thermophilic bacterium Thermus aquaticus has been purified to homogeneity. The enzyme is pyridoxal-5-phosphate dependent and is composed of two subunits having an Mr 44000 each. T. aquaticus AspAT shows a pI of 4.5 and is a thermophilic enzyme exhibiting its maximum activity at 80 degrees C in the pH range 7-8. It is also resistant against denaturation to heat and several chemical agents.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11367/28084
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 0
  • ???jsp.display-item.citation.isi??? 0
social impact