An ecotoxicological approach is used to assess the efficiency of an in-batch bioremediation process in reducing environmental toxicity of a soil polluted with Polycyclic Aromatic Hydrocarbons (PAHs). Microbial strains, able to use PAHs as a sole source of carbon, were added to a soil artificially contaminated with naphthalene, anthracene, phenanthrene, pyrene, and benzo[a]pyrene, with concentrations ranging from 0.4 to 0.1 mg/g. A phytoxicity test (Lepidium sativum) a chronic assay (Ceriodaphnia dubia) and acute assays (Daphnia magna, Artemia salina and Ceriodaphnia dubia) were performed after by incubating soil spiked with selected microbial strains and PHAs for 2 months. PAHs concentration was measured monthly by High Pressure Liquid Cromatography. A decrement of PHAs was observed as result of microbial metabolism. The obtained data showed a positive correlation with the decrement of PAHs for acute and phytotoxicity tests, while an opposite result was observed for chronic assays. The opportunity to implement ecot