The stability against chemical denaturants of the elongation factor EF-1A (SsEF-1A), a protein isolated from the hyperthermophilic archaeon Sulfolobus solfataricus has been characterized in detail. Indeed, the atypical shape of the protein structure and the unusual living conditions of the host organism prompted us to analyze the effect of urea and guanidine hydrochloride (GuHCl) on the GDP complex of the enzyme (SsEF-1•GDP) by fluorescence and circular dichroism. These studies were also extended to the nucleotide-free form of the protein (nfSsEF-1A). Interestingly, the experiments show that the denaturation curves of both SsEF-1A forms present a single inflection point, which is indicative of a cooperative unfolding process with no intermediate species. Moreover, the chemically induced unfolding process of both SsEF- 1A•GDP and nfSsEF-1A is fully reversible. Both SsEF-1A forms exhibit remarkable stability against urea, but they do not display a strong resistance to the denaturing action of GuHCl. These findings suggest that electrostatic interactions significantly contribute to SsEF-1A stability.

Chemical Denaturation of the Elongation Factor 1A Isolated from the Hyperthermophilic Archaeon Sulfolobus solfataricus

MASULLO, Mariorosario;
2006

Abstract

The stability against chemical denaturants of the elongation factor EF-1A (SsEF-1A), a protein isolated from the hyperthermophilic archaeon Sulfolobus solfataricus has been characterized in detail. Indeed, the atypical shape of the protein structure and the unusual living conditions of the host organism prompted us to analyze the effect of urea and guanidine hydrochloride (GuHCl) on the GDP complex of the enzyme (SsEF-1•GDP) by fluorescence and circular dichroism. These studies were also extended to the nucleotide-free form of the protein (nfSsEF-1A). Interestingly, the experiments show that the denaturation curves of both SsEF-1A forms present a single inflection point, which is indicative of a cooperative unfolding process with no intermediate species. Moreover, the chemically induced unfolding process of both SsEF- 1A•GDP and nfSsEF-1A is fully reversible. Both SsEF-1A forms exhibit remarkable stability against urea, but they do not display a strong resistance to the denaturing action of GuHCl. These findings suggest that electrostatic interactions significantly contribute to SsEF-1A stability.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11367/20160
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