C-reactive protein (CRP) is a major acute phase reactant in man but not in mouse. It is synthesized in abundant quan- tities by human hepatocytes during the course of several diseases, mainly acute inflammations. To investigate the regulation of CRP expression, the human CRP gene was introduced into fertilized eggs by microinjection and trans- genic mouse lines were derived. The CRP gene is exclusively transcribed in the liver and expression is strictly dependent on experimental inflammation. The kinetics of induction both for RNA and protein synthesis is very fast; RNA is first detectable after 2 h in the liver, the protein after 6 h in the serum. Human CRP levels in the sera of transgenic mice are com- parable to those observed in human diseases. Nuclear run- on experiments indicate that regulation is primarily at the transcriptional level.
Inducible and tissue-specific expression of human C-reactive protein in transgenic mice
ARCONE, Rosaria;
1987-01-01
Abstract
C-reactive protein (CRP) is a major acute phase reactant in man but not in mouse. It is synthesized in abundant quan- tities by human hepatocytes during the course of several diseases, mainly acute inflammations. To investigate the regulation of CRP expression, the human CRP gene was introduced into fertilized eggs by microinjection and trans- genic mouse lines were derived. The CRP gene is exclusively transcribed in the liver and expression is strictly dependent on experimental inflammation. The kinetics of induction both for RNA and protein synthesis is very fast; RNA is first detectable after 2 h in the liver, the protein after 6 h in the serum. Human CRP levels in the sera of transgenic mice are com- parable to those observed in human diseases. Nuclear run- on experiments indicate that regulation is primarily at the transcriptional level.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
